Dmitry Mazurov was born on April 13, 1981, in Novosibirsk. His aspiration for acting manifested in Dmitry already in early childhood. During his school years, he enrolled in the "Studio of Plastic Arts," created at the Novosibirsk Youth Academic Theater "Globus," which at that time was led by Georgy Vyacheslavovich Erasek. As part of his training, Mazurov took an extremely active part in children's productions. In 1998, after graduating from school, he submitted documents to the Novosibirsk Theater School. The entrance exams went successfully, and the young man was accepted into the course of Honored Artists Alexander Kuznetsov and Tamara Kocherzhinskaya. Throughout his entire student period, Mazurov continued to perform at the "Globus" theater. By 2002, he had already taken the stage in such graduation productions as "The Two Gentlemen of Verona" based on William Shakespeare's comedy (the role of Valentine), "The Lower Depths" based on Maxim Gorky's drama of the same name (the role of Kleshch), "The Staircase" based on Lyudmila Petrushevskaya's play, as well as "The Wife" based on Chekhov's work (the role of Nikolai Yevgrafych).

Mazurov decided to continue his studies in acting in the capital and, after moving to Moscow, was admitted directly to the third year at the All-Russian State Institute of Cinematography named after S. A. Gerasimov. The aspiring actor was enrolled in the workshop of Vitaly Mefodyevich Solomin and Alexander Sergeyevich Lenkov, and just two years later received a second diploma of higher education. Dmitry's examination works this time were the lead role in Molière's production "Tartuffe, or The Impostor" and the role of Pertti Oras in the comedy by Finnish playwright Ensio Rislakki "The Ugly Elsa." Mazurov's film debut took place in an episode of Pyotr Stein's domestic historical television novel "Poor Nastya," starring Elena Korikova and Daniil Strakhov in the lead roles. Then the actor appeared as Gosha Rokhlin in one episode of the detective series "Opera-1. Chronicles of the Homicide Department" (the episode "Who Wants to Be a Millionaire"). In 2005, Dmitry appeared in the continuation of Vyacheslav Murugov's comedy project "Soldiers-4," where he played the contract soldier Seryoga. At the same time, he worked on the series by Dmitry Kuzmin, Svetlana Lopatukhina, and Boris Kazakov "RublevkaLive."

In 2005, Mazurov's filmography was expanded with two new television projects – "Doomed to Become a Star" and "Hunting for the Izubr." A couple of years later, Oleg Ryaskov's film "The Sovereign's Servant" was released on screens with Dmitry Miller and Valery Malikov in the lead roles, and Dmitry himself appeared in the image of a servant. A year later, he took part in the work on Vartan Akopyan's comedy "Platon." At the same time, the actor was filming in the popular sitcom about student life "Univer" directed by Pyotr Tochilin. In 2009, Dmitry appeared in the image of an NKVD soldier in Nikita Mikhalkov's film "Burnt by the Sun 2." Filming in series continued with the release of Vyacheslav Murugov's successful project "Daddy's Daughters." In 2011, Mazurov performed one of the key roles in Ilya Kulikov's television series "Pyatnitsky," as well as in its continuation "Karpov" (2012).