Jennifer Lim studied at the Yale School of Drama, where she earned an MFA in acting. She is half Chinese and half Korean and is fluent in Cantonese and Mandarin (as well as basic Korean). She made her Broadway debut in the lead role in David Henry Hwang's play Chinglish and has worked in Off-Broadway theaters, as well as many prominent theaters in central New York and regional theaters.

In addition to her stage work, Jennifer has appeared in film and on television, especially in television police procedurals.