Jefferson Mays (born Lewis Jefferson Mays; June 8, 1965) is an American film, stage, and television actor.

Mays grew up in Clinton, Connecticut, with his parents — a naval intelligence officer and a children's librarian — and his siblings. Mays graduated from Yale College, where he earned a Bachelor of Arts degree, and from the University of California, San Diego, where he earned a Master of Fine Arts degree.

From November 2003 (previews) through October 31, 2004, Mays performed on Broadway in I Am My Own Wife, Douglas Wright's Pulitzer Prize-winning play. He had previously appeared in the play Off-Broadway at Playwrights Horizons in May 2003 and at La Jolla Playhouse in July 2001.

For his solo performance, Mays received the 2004 Tony Award for Best Actor in a Play, the 2004 Drama Desk Award for Outstanding Solo Show, an Obie Award, and the 2004 Theatre World Award. He also received the 2007 Helpmann Award for Best Actor in a Play for the 2006 Australian run of I Am My Own Wife.